2017 Transposon Insertion Sequencing Workshop

Instructor: Mark Mandel

Dates: July 17-19, 2017

Day 1: Morning (9:30 am - 12:30 pm)

Historical context for INSeq

Considerations for experimental design

Transposon selection and design

Day 1: Afternoon (1:30 pm - 4:30 pm)

Hands-on design steps for transposon vector(s)

Hands-on library review of library preparation protocol

Day 2: Morning (9:30 am - 12:30 pm)

Command line refresher

Logging into the cloud (XSEDE Jetstream) - Thanks to the DIB Lab for these materials

Day 2: Afternoon (1:30 pm - 4:30 pm)

Installing the pyinseq pipeline

Running the data analysis pipeline

Day 3: Morning (10:30 am - 12:30 pm)

Review results, rerun samples

Data deposition

Day 3: Afternoon (1:30 pm - 3:00 pm)

Variations on the theme: alternate options to consider

Wrap-up

Resources

Workshop docs rendered

Workshop docs source

DIB Summer Institute