LOCUS       pMarVF1   4582 BP DS-DNA   CIRCULAR  SYN       04-SEP-2012
DEFINITION  -
ACCESSION   -
KEYWORDS    -
SOURCE      -
FEATURES             Location/Qualifiers
     CDS             complement(1..861)
                     /note="bla"
     promoter        complement(896..902)
                     /note="P3"
     misc_feature    complement(3753..3756)
                     /note="MCS"
     misc_feature    complement(3773..4546)
                     /note="RP4/oriT/oriR6K"
     gene            2698..3744
                     /note="himar1C9"
     frag            1078..1106
                     /note="MmeI IR_L"
     frag            2363..2391
                     /note="MmeI IR_R"
     terminator      2115..2158
                     /note="E. coli rrnB terminator T1"
     terminator      2290..2317
                     /note="E. coli rrnB terminator T2"
     misc_feature    1078..2391
                     /note="Transposon"
     CDS             1341..2078
                     /gene="ErmR"
     insert          2393..2696
                     /source="nrdR (VF_0699) promoter"
ORIGIN      
          1 TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT
         61 AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC
        121 CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA
        181 CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA
        241 GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA
        301 CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT
        361 CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC
        421 GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT
        481 CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC
        541 TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG
        601 CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT
        661 CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC
        721 CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG
        781 CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC
        841 ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG
        901 TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT
        961 TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGTCTAA GAAACCATTA TTATCATGAC
       1021 ATTAACCTAT AAAAATAGGC GTATCACGAG GCCCTTTCGT CACGCGTCCT CGGTACCTAA
       1081 CAGGTTGGAT GATAAGTCCC CGGTCTTCGT ATGCCGTCTT CTGCTTGGCG CGCCCtcgag
       1141 GGTTCGTGTT CGTGCTGACT TGCACCATAT CATAAAAATC GAAACAGCAA AGAATGGCGG
       1201 AAACGTAAAA GAAGTTATGG AAATAAGACT TAGAAGCAAA CTTAAGAGTG TGTTGATAGT
       1261 GCAGTATCTT AAAATTTTGT ATAATAGGAA TTGAAGTTAA ATTAGATGCT AAAAATTTGT
       1321 AATTAAGAAG GAGTGATTAC ATGAACAAAA ATATAAAATA TTCTCAAAAC TTTTTAACGA
       1381 GTGAAAAAGT ACTCAACCAA ATAATAAAAC AATTGAATTT AAAAGAAACC GATACCGTTT
       1441 ACGAAATTGG AACAGGTAAA GGGCATTTAA CGACGAAACT GGCTAAAATA AGTAAACAGG
       1501 TAACGTCTAT TGAATTAGAC AGTCATCTAT TCAACTTATC GTCAGAAAAA TTAAAACTGA
       1561 ATACTCGTGT CACTTTAATT CACCAAGATA TTCTACAGTT TCAATTCCCT AACAAACAGA
       1621 GGTATAAAAT TGTTGGGAGT ATTCCTTACC ATTTAAGCAC ACAAATTATT AAAAAAGTGG
       1681 TTTTTGAAAG CCATGCGTCT GACATCTATC TGATTGTTGA AGAAGGATTC TACAAGCGTA
       1741 CCTTGGATAT TCACCGAACA CTAGGGTTGC TCTTGCACAC TCAAGTCTCG ATTCAGCAAT
       1801 TGCTTAAGCT GCCAGCGGAA TGCTTTCATC CTAAACCAAA AGTAAACAGT GTCTTAATAA
       1861 AACTTACCCG CCATACCACA GATGTTCCAG ATAAATATTG GAAGCTATAT ACGTACTTTG
       1921 TTTCAAAATG GGTCAATCGA GAATATCGTC AACTGTTTAC TAAAAATCAG TTTCATCAAG
       1981 CAATGAAACA CGCCAAAGTA AACAATTTAA GTACCGTTAC TTATGAGCAA GTATTGTCTA
       2041 TTTTTAATAG TTATCTATTA TTTAACGGGA GGAAATAAtC TAGAATGCGA GAGTAGGGAA
       2101 CTGCCAGGCA TCAAATAAAA CGAAAGGCTC AGTCGAAAGA CTGGGCCTTT CGTTTTATCT
       2161 GTTGTTTGTC GGTGAACGCT CTCCTGAGTA GGACAAATCC GCCGGGAGCG GATTTGAACG
       2221 TTGCGAAGCA ACGGCCCGGA GGGTGGCGGG CAGGACGCCC GCCATAAACT GCCAGGCATC
       2281 AAATTAAGCA GAAGGCCATC CTGACGGATG GCCTTTTTGC GTTTCTACCT GCAGGGCGCG
       2341 CCAAGCAGAA GACGGCATAC GAAGACCGGG GACTTATCAT CCAACCTGTT AGGATCCgta
       2401 attggcatca taaacatttt cattttcttg taataacagt tgaaataaga atgttgagat
       2461 aatcaatgtc ggtagaagtg atatgattaa taatcgatgt tttatacttt taaatacaga
       2521 caaaatcata gttaaccttt taattaatgt gggtatatac atttatcggt tgcattaatt
       2581 gatactttaa taattaaaac tgggtttttc ttttggttta aacaggaact taatgaaggg
       2641 acataggcag gcatctgctt ttcttgtaca ctaattgaaa aaactaggag gcaaCATATG
       2701 GAAAAAAAGG AATTTCGTGT TTTGATAAAA TACTGTTTTC TGAAGGGAAA AAATACAGTG
       2761 GAAGCAAAAA CTTGGCTTGA TAATGAGTTT CCGGACTCTG CCCCAGGGAA ATCAACAATA
       2821 ATTGATTGGT ATGCAAAATT CAAGCGTGGT GAAATGAGCA CGGAGGACGG TGAACGCAGT
       2881 GGACGCCCGA AAGAGGTGGT TACCGACGAA AACATCAAAA AAATCCACAA AATGATTTTG
       2941 AATGACCGTA AAATGAAGTT GATCGAGATA GCAGAGGCCT TAAAGATATC AAAGGAACGT
       3001 GTTGGTCATA TCATTCATCA ATATTTGGAT ATGCGGAAGC TCTGTGCGAA ATGGGTGCCG
       3061 CGCGAGCTCA CATTTGACCA AAAACAACGA CGTGTTGATG ATTCTAAGCG GTGTTTGCAG
       3121 CTGTTAACTC GTAATACACC CGAGTTTTTC CGTCGATATG TGACAATGGA TGAAACATGG
       3181 CTCCATCACT ACACTCCTGA GTCCAATCGA CAGTCGGCTG AGTGGACAGC GACCGGTGAA
       3241 CCGTCTCCGA AGCGTGGAAA GACTCAAAAG TCCGCTGGCA AAGTAATGGC CTCTGTTTTT
       3301 TGGGATGCGC ATGGAATAAT TTTTATCGAT TATCTTGAGA AGGGAAAAAC CATCAACAGT
       3361 GACTATTATA TGGCGTTATT GGAGCGTTTG AAGGTCGAAA TCGCGGCAAA ACGGCCCCAC
       3421 ATGAAGAAGA AAAAAGTGTT GTTCCACCAA GACAACGCAC CGTGCCACAA GTCATTGAGA
       3481 ACGATGGCAA AAATTCATGA ATTGGGCTTC GAATTGCTTC CCCACCCGCC GTATTCTCCA
       3541 GATCTGGCCC CCAGCGACTT TTTCTTGTTC TCAGACCTCA AAAGGATGCT CGCAGGGAAA
       3601 AAATTTGGCT GCAATGAAGA GGTGATCGCC GAAACTGAGG CCTATTTTGA GGCAAAACCG
       3661 AAGGAGTACT ACCAAAATGG TATCAAAAAA TTGGAAGGTC GTTATAATCG TTGTATCGCT
       3721 CTTGAAGGGA ACTATGTTGA ATAAGCGGCC GCCACCGCGG TGGAGGGGAA TTCCCATGTC
       3781 AGCCGTTAAG TGTTCCTGTG TCACTCAAAA TTGCTTTGAG AGGCTCTAAG GGCTTCTCAG
       3841 TGCGTTACAT CCCTGGCTTG TTGTCCACAA CCGTTAAACC TTAAAAGCTT TAAAAGCCTT
       3901 ATATATTCTT TTTTTTCTTA TAAAACTTAA AACCTTAGAG GCTATTTAAG TTGCTGATTT
       3961 ATATTAATTT TATTGTTCAA ACATGAGAGC TTAGTACGTG AAACATGAGA GCTTAGTACG
       4021 TTAGCCATGA GAGCTTAGTA CGTTAGCCAT GAGGGTTTAG TTCGTTAAAC ATGAGAGCTT
       4081 AGTACGTTAA ACATGAGAGC TTAGTACGTG AAACATGAGA GCTTAGTACG TACTATCAAC
       4141 AGGTTGAACT GCTGATCTTC AGATCCTCTA CGCCGGACGC ATCGTGGCCG GATCAATTCC
       4201 GTTTTCCGCT GCATAACCCT GCTTCGGGGT CATTATAGCG ATTTTTTCGG TATATCCATC
       4261 CTTTTTCGCA CGATATACAG GATTTTGCCA AAGGGTTCGT GTAGACTTTC CTTGGTGTAT
       4321 CCAACGGCGT CAGCCGGGCA GGATAGGTGA AGTAGGCCCA CCCGCGAGCG GGTGTTCCTT
       4381 CTTCACTGTC CCTTATTCGC ACCTGGCGGT GCTCAACGGG AATCCTGCTC TGCGAGGCTG
       4441 GCCGGCTACC GCCGGCGTAA CAGATGAGGG CAAGCGGATG GCTGATGAAA CCAAGCCAAC
       4501 CAGGAAGGGC AGCCCACCTA TCACGGAATT GATCCCCCTC GAATTGACGC GT
//

